Biotechnological approaches and Micropropagation in Vegetable Crops
Course Overview
Official detailed syllabus from the ICAR Sixth Deans’ Committee curriculum.
Course Objectives
To educate students about latest biotechnical advancements in vegetables
Theory Syllabus
Concepts and applications of plant biotechnology. Introduction to recombinant DNA methods: physical, chemical and Agrobacterium mediated gene transfer methods; Transgenics and its importance in crop improvement; PCR techniques and its applications; DNA markers and their application - RFLP, RAPD, AFLP, CAPS, SSR etc. Marker Assisted Breeding in crop improvement; Biotechnology regulations. Plant Cell and Tissue Culture - organ culture, embryo culture, cell suspension culture, protoplast culture, callus culture, anther culture, pollen culture, ovule culture and their applications; Micro-propagation methods; organogenesis and embryogenesis, Synthetic seeds and their significance; Embryo rescue and its significance. Somatic hybridization and cybrids. Soma-clonal variation and its use in crop improvement. Cryo-preservation. Application of in-vitro techniques.
Practical Syllabus
Preparation of solution, pH and buffers. Sterilization techniques. Composition of various tissue culture media and preparation of stock solutions for MS nutrient medium. Callus induction from various explants and plant regeneration. Micro-propagation, hardening and acclimatization. Demonstration on isolation of DNA and PCR technique. Demonstration of gel electrophoresis techniques and DNA finger printing.
Suggested Readings
1. Bajaj YPS (Ed.). 1987. Biotechnology in Agriculture and Forestry. Vol. XIX. Hitech and Micropropagation. Springer.
2. Chadha K L, Ravindran P N and Sahijram L (Eds). 2000. Biotechnology of Horticulture and Plantation Crops. Malhotra Publ. House.
3. Debnath M. 2005. Tools and Techniques of Biotechnology. Pointer Publication. New Delhi. Horticultural Sciences–Vegetable Science
4. Keshavachandran R. 2007. Recent Trends in Biotechnology of Horticultural Crops. New India Publ. Agency.
5. Keshavachandran R and Peter K V. 2008. Plant Biotechnology; Tissue Culture and Gene Transfer. Orient and Longman, USA.
6. Keshavachandran R. 2007. Recent Trends in Biotechnology of Horticultural Crops. New-India Publication Agency, New Delhi.
7. Parthasarathy V A, Bose T K, Deka P C, Das P, Mitra S K and Mohanadas S. 2001. Biotechnology of Horticultural Crops. Vols. I-III. Naya Prokash. Pierik RLM. 1987. In-vitro Culture of Higher Plants. Martinus Nijhoff Publ.
8. Prasad S. 1999. Impact of Plant Biotechnology on Horticulture. (2nd Edn.). Agro Botanica.
9. Rout G R and Peter K V. 2018. Genetic Engineering of Horticultural Crops. Academic Press Elsevier, USA.
10. Sharma R. 2000. Plant Tissue Culture. Campus Books.
11. Singh B D. 2010. Biotechnology- Expanding Horizons. Kalyani Publishers, New Delhi.
12. Vasil T K, Vasi M, While DNR and Bery H R. 1979. Somatic Hybridization and Genetic Manipulation in Plants, Plant Regulation and World Agriculture. Planum Press.